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SEC61A1 antibody, C-term

Anti-SEC61A1 antibody, C-term used in Western Blot (WB). GTX89964
Anti-SEC61A1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89964

Cat. No. GTX89964

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all SEC61A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89964-PEP).

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KEQSEVGSMGALLF, from the C Terminus of the protein sequence according to NP_037468.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sec61 translocon alpha 1 subunit , HNFJ4 , HSEC61 , SEC61 , SEC61A

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SEC61A1 antibody, C-term used in Western Blot (WB). GTX89964

GTX89964 WB Image

WB analysis of human brain (cerebellum) lysate using GTX89964 SEC61A1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-SEC61A1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89964

GTX89964 IHC-P Image

IHC-P analysis of human liver using GTX89964 SEC61A1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399