APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
K562 membrane fraction extract (un-boiled)
Predict Reactivity
Mouse, Rat, Zebrafish, Bovine, Dog, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SEC61A1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241302
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Sec61 translocon alpha 1 subunit , HNFJ4 , HSEC61 , SEC61 , SEC61A
Cellular Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein
Background
The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX106455 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC61A1(GTX106455) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX106455 ICC/IF Image
Immunofluorescence analysis of methanol-fixed A431, using SEC61A1(GTX106455) antibody at 1:200 dilution.
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GTX106455 WB Image
SEC61A1 antibody detects SEC61A1 protein by Western blot analysis. K562 whole cell extracts and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC61A1 antibody (GTX106455) diluted at 1:1000.
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REFERENCE
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REVIEW
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