GeneTex
United States (US)

SEC61A1 antibody

Orthogonal
GTX106455_39694_IFA_19011506_403.jpg
GTX106455_IHC_19011506_274.jpg
GTX106455_42018_20150903_WB_Fraction_19011506_661.jpg

Cat No. GTX106455

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

K562 whole cell extract , K562 membrane fraction extract (un-boiled)

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SEC61A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HSEC61 antibody, SEC61 antibody, SEC61A antibody, SEC61A1 antibody, protein transport protein SEC61 alpha subunit antibody, sec61 homolog antibody, Sec61 alpha-1 antibody, protein transport protein Sec61 subunit alpha antibody, protein transport protein Sec61 subunit alpha isoform 1 antibody, Sec61 alpha 1 subunit (S. cerevisiae) antibody

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein

Background

The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq]

Database

DATA IMAGES
GTX106455_39694_IFA_19011506_403.jpg

GTX106455 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using SEC61A1(GTX106455) antibody at 1:200 dilution.

GTX106455_IHC_19011506_274.jpg

GTX106455 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC61A1(GTX106455) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106455_42018_20150903_WB_Fraction_19011506_661.jpg

GTX106455 WB Image

Orthogonal

SEC61A1 antibody detects SEC61A1 protein by Western blot analysis. K562 whole cell extracts and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC61A1 antibody (GTX106455) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169