GeneTex
United States (US)

SEC61B antibody

GTX15576_270_ICC-IF_19011519_292.jpg

Cat No. GTX15576

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat, Dog, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:20,000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Specificity/Sensitivity

This antibody shows no cross-reactivity with other sec61 isoforms.
PROPERTIES

Form

Liquid

Buffer

Serum, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Dog

Immunogen

Synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sec61 Translocon Beta Subunit,Sec61B

Cellular Localization

Endoplasmic reticulum membrane

Background

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX15576_270_ICC-IF_19011519_292.jpg

GTX15576 ICC/IF Image

ICC/IF analysis of MDCK cells using GTX15576 SEC61B antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

Package List Price ($)
$ 319