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SEC62 antibody

Anti-SEC62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31455
Anti-SEC62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31455
Anti-SEC62 antibody used in Western Blot (WB). GTX31455

Cat No. GTX31455

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

Rat Brain Tissue Lysate(GTX27942)

Specificity/Sensitivity

SEC62 antibody is predicted to not cross-react with other SEC proteins. Multiple isoforms of SEC62 are known to exist.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SEC62 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SEC62.The immunogen is located within amino acids 270 - 320 of SEC62.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SEC62 homolog, preprotein translocation factor , Dtrp1 , HTP1 , TLOC1 , TP-1

Cellular Localization

Endoplasmic reticulum membrane

Background

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-SEC62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31455

GTX31455 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31455 SEC62 antibody.
Working concentration : 5 μg/ml

Anti-SEC62 antibody used in IHC (Paraffin sections) (IHC-P). GTX31455

GTX31455 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31455 SEC62 antibody.
Working concentration : 20 μg/ml

Anti-SEC62 antibody used in Western Blot (WB). GTX31455

GTX31455 WB Image

WB analysis of rat brain tissue lysate using GTX31455 SEC62 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339