IHC-P analysis of human lung tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

FACS analysis of HEK293T cells transfected with either SEK1 / MKK4 plasmid(Red) or empty vector control plasmid(Blue) using GTX84161 SEK1 / MKK4 antibody [4B4].

IP analysis of DDDDK tagged SEK1 / MKK4 overexpressed HEK293T lysate using GTX84161 SEK1 / MKK4 antibody [4B4].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of HEK293T cells transfected with SEK1 / MKK4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84161 SEK1 / MKK4 antibody [4B4].
Loading : 5 ug per lane

IHC-P analysis of human breast tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human colon tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human kidney tissue using GTX84161 SEK1 / MKK4 antibody [4B4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84161 SEK1 / MKK4 antibody [4B4].