GeneTex
United States (US)

SEK1 / MKK4 antibody

KO/KD
GTX35224_20180822_WB_1_18121410_524.jpg
GTX35224_20180822_WB_18121410_280.jpg

Cat No. GTX35224

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MAP2K4 (NP_003001.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase 4,Jnkk,Jnkk1,Mapkk4,Mek4,Mkk4,Prkmk4,Sapkk-1,Sapkk1,Sek1,Serk1,Skk1,Map2K4

Background

This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver. [provided by RefSeq, Jul 2008]
DATA IMAGES
GTX35224_20180822_WB_1_18121410_524.jpg

GTX35224 WB Image

KO/KD

WB analysis of wild type and SEK1 / MKK4 knockout cell lysate using GTX35224 SEK1 / MKK4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX35224_20180822_WB_18121410_280.jpg

GTX35224 WB Image

KO/KD

WB analysis of various samples using GTX35224 SEK1 / MKK4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329