GeneTex
United States (US)

SEK1 / MKK4 (phospho Thr261) antibody

GTX32260_20190131_WB_19013101_933.jpg
GTX32260_20190131_IHC-P_19013101_453.jpg
GTX32260_20190131_ICCIF_19013101_317.jpg

Cat No. GTX32260

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

44 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of SEK1 / MKK4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase 4,Jnkk,Jnkk1,Mapkk4,Mek4,Mkk4,Prkmk4,Sapkk-1,Sapkk1,Sek1,Serk1,Skk1,Map2K4

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES
GTX32260_20190131_WB_19013101_933.jpg

GTX32260 WB Image

WB analysis of PDGF-treated NIH3T3 (A) whole cell lysates using GTX32260 SEK1 / MKK4 (phospho Thr261) antibody.

GTX32260_20190131_IHC-P_19013101_453.jpg

GTX32260 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32260 SEK1 / MKK4 (phospho Thr261) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32260_20190131_ICCIF_19013101_317.jpg

GTX32260 ICC/IF Image

ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX32260 SEK1 / MKK4 (phospho Thr261) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Package List Price ($)
$ 319