*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Mouse, Rat(>80% identity)
PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide sequence around phosphorylation site of threonine 261 (A-K-T(p)-RD) derived from human SEK1 / MKK4.
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
mitogen-activated protein kinase kinase 4 , JNKK , JNKK1 , MAPKK4 , MEK4 , MKK4 , PRKMK4 , SAPKK-1 , SAPKK1 , SEK1 , SERK1 , SKK1
This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
GTX50226 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX50226 SEK1 / MKK4 (phospho Thr261) antibody.
GTX50226 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50226 SEK1 / MKK4 (phospho Thr261) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide