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SEK1 / MKK4 (phospho Thr261) antibody

Anti-SEK1 / MKK4 (phospho Thr261) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50226
Anti-SEK1 / MKK4 (phospho Thr261) antibody used in IHC (Paraffin sections) (IHC-P). GTX50226

Cat No. GTX50226

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 261 (A-K-T(p)-RD) derived from human SEK1 / MKK4.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase kinase 4 , JNKK , JNKK1 , MAPKK4 , MEK4 , MKK4 , PRKMK4 , SAPKK-1 , SAPKK1 , SEK1 , SERK1 , SKK1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-SEK1 / MKK4 (phospho Thr261) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50226

GTX50226 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50226 SEK1 / MKK4 (phospho Thr261) antibody.

Anti-SEK1 / MKK4 (phospho Thr261) antibody used in IHC (Paraffin sections) (IHC-P). GTX50226

GTX50226 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50226 SEK1 / MKK4 (phospho Thr261) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for SEK1 / MKK4 (phospho Thr261) antibody (GTX50226). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SEK1 / MKK4 (phospho Thr261) antibody (GTX50226). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf