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SELK antibody

Anti-SELK antibody used in IHC (Paraffin sections) (IHC-P). GTX32088
Anti-SELK antibody used in Western Blot (WB). GTX32088

Cat. No. GTX32088

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all SELK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

A20 Cell Lysate(GTX27180) , Human Heart Tissue Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SELK antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SELK.The immunogen is located within the last 50 amino acids of SELK.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

selenoprotein K , HSPC030 , HSPC297 , SELK

Cellular Localization

Endoplasmic reticulum membrane,Cell membrane

Background

This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is localized to the endoplasmic reticulum and is highly expressed in the heart, where it may function as an antioxidant. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SELK antibody used in IHC (Paraffin sections) (IHC-P). GTX32088

GTX32088 IHC-P Image

IHC-P analysis of human heart tissue using GTX32088 SELK antibody.
Working concentration : 20 μg/ml

Anti-SELK antibody used in Western Blot (WB). GTX32088

GTX32088 WB Image

WB analysis of A20 cell lysate using GTX32088 SELK antibody.
Dilution : 1μg/ml (Lane A) and 2 μg/ml (Lane B)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399