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SEMA4B antibody

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035
Anti-SEMA4B antibody used in Western Blot (WB). GTX121035
Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035
Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035
Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035
Anti-SEMA4B antibody used in Western Blot (WB). GTX121035

Cat No. GTX121035

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , MCF-7 , mouse brain

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SEMA4B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731613

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

semaphorin 4B , SEMAC , SemC

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035

GTX121035 IHC-P Image

SEMA4B antibody detects SEMA4B protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEMA4B antibody used in Western Blot (WB). GTX121035

GTX121035 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX121035 diluted at 1:1000

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035

GTX121035 IHC-P Image

SEMA4B antibody detects SEMA4B protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035

GTX121035 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using SEMA4B(GTX121035) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEMA4B antibody used in IHC (Paraffin sections) (IHC-P). GTX121035

GTX121035 IHC-P Image

SEMA4B antibody detects SEMA4B protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEMA4B antibody used in Western Blot (WB). GTX121035

GTX121035 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX121035 diluted at 1:1000

REFERENCE

REVIEW

SEMA4B antibody Cat No. GTX121035

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29311693
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018.PMID: 29311693
SDS
Glycerol.pdf
Thimerosal.pdf