SEMA4B antibody detects SEMA4B protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SEMA4B antibody detects SEMA4B protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX121035 diluted at 1:1000

SEMA4B antibody detects SEMA4B protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
SEMA4B antibody (GTX121035) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using SEMA4B(GTX121035) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA4B antibody (GTX121035) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Sci Rep in 2018.PMID: 29311693

The data was published in the journal Sci Rep in 2018.PMID: 29311693