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SEMA4F antibody, N-term

KO/KD
Anti-SEMA4F antibody, N-term used in Western Blot (WB). GTX46442
Anti-SEMA4F antibody, N-term used in Western Blot (WB). GTX46442
Anti-SEMA4F antibody, N-term used in Western Blot (WB). GTX46442

Cat. No. GTX46442

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
50 μg ($399)
See all SEMA4F products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

A431 , MIA-PACA2 , Transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human SEMA4F

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ssemaphorin 4F , M-SEMA , PRO2353 , S4F , SEMAM , SEMAW , m-Sema-M

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a transmembrane class IV semaphorin family protein, which plays a role in neural development. This gene may be involved in neurogenesis in prostate cancer, the development of neurofibromas, and breast cancer tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-SEMA4F antibody, N-term used in Western Blot (WB). GTX46442

GTX46442 WB Image

WB analysis of MIA-PACA2 cells over-express human SEMA4F protein using SEMA4F antibody (GTX46442)
Lanes:
1. Untransfected MIA-PACA2 cell lysate
2. Untransfected MIA-PACA2 cell lysate
3. Untransfected MIA-PACA2 cell lysate
4. MIA-PACA2 cells over-expressing human SEMA4F
5. Untransfected MIA-PACA2 cell lysate
6. MIA-PACA2 cells over-expressing human SEMA4F

Anti-SEMA4F antibody, N-term used in Western Blot (WB). GTX46442

GTX46442 WB Image

KO/KD

WB analysis of A431 cells using GTX46442 SEMA4F antibody at 1:600.
Lane 1 (-): A431 cells transfected with control siRNA
Lane 2 (+): A431 cells transfected with Sema4F siRNA

Anti-SEMA4F antibody, N-term used in Western Blot (WB). GTX46442

GTX46442 WB Image

WB analysis of tranfected SEMA4F (isoform 2) 293T cells using GTX46442 SEMA4F antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399