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SEMA6A antibody [HL2120]

Recombinant antibody Orthogonal
Anti-SEMA6A antibody [HL2120] used in Western Blot (WB). GTX638092
Anti-SEMA6A antibody [HL2120] used in Western Blot (WB). GTX638092
Anti-SEMA6A antibody [HL2120] used in Western Blot (WB). GTX638092

Cat. No. GTX638092

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2120

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SEMA6A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Product Note

This antibody was raised against human SEMA6A Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human SEMA6A. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

semaphorin 6A , HT018 , SEMA , SEMA6A1 , SEMAQ , VIA

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al., 2001 [PubMed 11242070]).[supplied by OMIM, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-SEMA6A antibody [HL2120] used in Western Blot (WB). GTX638092

GTX638092 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA6A antibody [HL2120] (GTX638092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 18187809

Anti-SEMA6A antibody [HL2120] used in Western Blot (WB). GTX638092

GTX638092 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA6A antibody [HL2120] (GTX638092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 18187809

Anti-SEMA6A antibody [HL2120] used in Western Blot (WB). GTX638092

GTX638092 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA6A antibody [HL2120] (GTX638092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations