GeneTex
United States (US)

SEMA6A antibody [N2C1], Internal

GTX105083_39953_WB_M_brain_19011506_965.jpg
GTX105083_39953_IFA_19011506_416.jpg
GTX105083_WB_19011506_371.jpg
GTX105083_39953_IHC_19011506_867.jpg

Cat No. GTX105083

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

A431 , HepG2 , mouse brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEMA6A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HT018 antibody, KIAA1368 antibody, SEMA antibody, SEMA6A1 antibody, SEMAQ antibody, VIA antibody, SEMA6A antibody, semaphorin 6A-1 antibody, semaphorin VIA antibody, semaphorin-6A antibody, SEMA6A-1 antibody, sema VIa antibody, semaphorin-6A-1 antibody, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Can act as repulsive axon guidance cues. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.
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Database

Research Area

DATA IMAGES
GTX105083_39953_WB_M_brain_19011506_965.jpg

GTX105083 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105083 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105083_39953_IFA_19011506_416.jpg

GTX105083 ICC/IF Image

SEMA6A antibody [N2C1], Internal detects SEMA6A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SEMA6A protein stained by SEMA6A antibody [N2C1], Internal (GTX105083) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX105083_WB_19011506_371.jpg

GTX105083 WB Image

SEMA6A antibody [N2C1], Internal detects SEMA6A protein by western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
B. 30 µg Molt-4 whole cell lysate/extract
C. 30 µg Raji whole lysate/extract
7.5% SDS-PAGE
SEMA6A antibody [N2C1], Internal (GTX105083) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105083_39953_IHC_19011506_867.jpg

GTX105083 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SEMA6A(GTX105083) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf