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SENP3 antibody

Anti-SENP3 antibody used in Western Blot (WB). GTX117938
Anti-SENP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX117938
Anti-SENP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117938

Cat. No. GTX117938

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SENP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Raji , K562 , THP-1

Predict Reactivity

Mouse, Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SENP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169137

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SUMO specific peptidase 3 , SMT3IP1 , SSP3 , Ulp1

Cellular Localization

Nucleus , nucleolus

Background

The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP3, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SENP3 antibody used in Western Blot (WB). GTX117938

GTX117938 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
B: K562
C: THP-1
7.5% SDS PAGE
GTX117938 diluted at 1:1000

Anti-SENP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX117938

GTX117938 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using SENP3(GTX117938) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SENP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117938

GTX117938 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SENP3(GTX117938) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169