APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1 μg/mL |
5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Raji Cell Slide(GTX25538) , Raji Cell Lysate(GTX27908)
Product Note
At least two isoforms of SEPT1 are known to exist. This antibody is predicted to not cross-react with other Septin protein family members.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
SEPT1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SEPT1.The immunogen is located within amino acids 300 - 350 of SEPT1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
septin 1 , DIFF6 , LARP , PNUTL3 , SEP1
Cellular Localization
Cytoplasm,Cytoplasm, cytoskeleton
Background
This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
Database
Research Area
DATA IMAGES
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GTX31484 ICC/IF Image
ICC/IF analysis of Raji cells using GTX31484 SEPT1 antibody. Working concentration : 5 μg/ml
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GTX31484 ICC/IF Image
ICC/IF analysis of Raji cells using GTX31484 SEPT1 antibody. Working concentration : 20 μg/ml
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GTX31484 WB Image
WB analysis of Raji cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31484 SEPT1 antibody. Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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