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SEPT1 antibody

Anti-SEPT1 antibody used in Western Blot (WB). GTX31484
Anti-SEPT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31484
Anti-SEPT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31484

Cat No. GTX31484

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL
ICC/IF 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Raji Cell Slide(GTX25538) , Raji Cell Lysate(GTX27908)

Specificity/Sensitivity

At least two isoforms of SEPT1 are known to exist. This antibody is predicted to not cross-react with other Septin protein family members.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SEPT1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SEPT1.The immunogen is located within amino acids 300 - 350 of SEPT1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

septin 1 , DIFF6 , LARP , PNUTL3 , SEP1

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-SEPT1 antibody used in Western Blot (WB). GTX31484

GTX31484 WB Image

WB analysis of Raji cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31484 SEPT1 antibody.
Working concentration : 1 μg/ml

Anti-SEPT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31484

GTX31484 ICC/IF Image

ICC/IF analysis of Raji cells using GTX31484 SEPT1 antibody.
Working concentration : 20 μg/ml

Anti-SEPT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31484

GTX31484 ICC/IF Image

ICC/IF analysis of Raji cells using GTX31484 SEPT1 antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339