APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.25 μg/ml |
| 2-5 μg/ml |
| 1-3 μg/1x10⁶ cells |
| 0.1-0.5 μg/ml |
|
Note :
IHC-P
Recommend EDTA buffer (pH 8.0) heat mediated antigen retrieval
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄
Preservative
No preservatives
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
E.coli-derived human SEPT6/SEPTIN6 recombinant protein (Position: M1-E434).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SEP2,SEPT2,SEPT6,septin 6,Septin 6
Cellular Localization
Cytoplasm,Cytoskeleton,Spindle,Chromosome,Centromere,Kinetochore,Cleavage furrow,Midbody,Cell projection,Cilium,Flagellum
Background
This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX04848 FACS Image
FACS analysis of ANA-1 cells using GTX04848 SEPT6 antibody.
Blue : Primary antibody.
Green : Isotype control.
Red : Unlabelled sample
Dilution : 1 μg/1x10⁶ cells
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GTX04848 FACS Image
FACS analysis of HEL cells using GTX04848 SEPT6 antibody.
Blue : Primary antibody.
Green : Isotype control.
Red : Unlabelled sample
Dilution : 1 μg/1x10⁶ cells
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GTX04848 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX04848 SEPT6 antibody.
Antigen Retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/ml
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GTX04848 WB Image
WB analysis of various samples using GTX04848 SEPT6 antibody.
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : MOLT-4 whole cell lysate
Lane 3 : rat thymus tissue lysate.
Lane 4 : rat brain tissue lysate
Lane 5 : mouse brain tissue lysate
Loading : 30 μg
Dilution : 0.25 μg/ml
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GTX04848 IHC-P Image
IHC-P analysis of human spleen tissue using GTX04848 SEPT6 antibody.
Antigen Retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/ml
|
REFERENCE
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REVIEW
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