GeneTex
United States (US)

SEPT7 antibody, Internal

GTX88524_2238_WB_18121411_240.jpg

Cat No. GTX88524

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88524-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all three reported isoforms (NP_001779.3; NP_001011553.2; NP_001229885.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig, Horse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DNNKNKGQLTKSP, from the internal region of the protein sequence according to NP_001779.3; NP_001011553.2; NP_001229885.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Septin 7,Cdc10,Cdc3,Nbla02942,Sept7A,Sept7

Cellular Localization

Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress

Background

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011]

Database

Research Area

DATA IMAGES
GTX88524_2238_WB_18121411_240.jpg

GTX88524 WB Image

WB analysis of HeLa nuclear lysate using GTX88524 SEPT7 antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329