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SEPT7 antibody [N3C3]

Anti-SEPT7 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX101533
Anti-SEPT7 antibody [N3C3] used in Western Blot (WB). GTX101533
Anti-SEPT7 antibody [N3C3] used in Western Blot (WB). GTX101533
Anti-SEPT7 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101533

Cat. No. GTX101533

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all SEPT7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEPT7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949551

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

septin 7 , CDC10 , CDC3 , NBLA02942 , SEPT7A

Cellular Localization

Cytoplasm , Kinetochore , Spindle

Background

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. Although the exact function of this gene has not yet been determined, its high similarity to yeast CDC10 and the high conservative nature of eukaryotic cell cycle machinery suggest a similar role to that of its yeast counterpart. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SEPT7 antibody [N3C3] used in IHC (Whole mount) (IHC-Wm). GTX101533

GTX101533 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using SEPT7 antibody [N3C3] (GTX101533) at 1:200 dilution.

Anti-SEPT7 antibody [N3C3] used in Western Blot (WB). GTX101533

GTX101533 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish brain
10% SDS PAGE
GTX101533 diluted at 1:1000

Anti-SEPT7 antibody [N3C3] used in Western Blot (WB). GTX101533

GTX101533 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101533 diluted at 1:1000

Anti-SEPT7 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101533

GTX101533 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using 39331(GTX101533) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169