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SEPT7 antibody [N3C3]

Anti-SEPT7 antibody [N3C3] used in Western Blot (WB). GTX101533
Anti-SEPT7 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101533

Cat No. GTX101533

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEPT7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

septin 7 , CDC10 , CDC3 , NBLA02942 , SEPT7A

Cellular Localization

Cytoplasm , Kinetochore , Spindle

Background

This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. Although the exact function of this gene has not yet been determined, its high similarity to yeast CDC10 and the high conservative nature of eukaryotic cell cycle machinery suggest a similar role to that of its yeast counterpart. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SEPT7 antibody [N3C3] used in Western Blot (WB). GTX101533

GTX101533 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101533 diluted at 1:1000

Anti-SEPT7 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101533

GTX101533 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using 39331(GTX101533) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for SEPT7 antibody [N3C3] (GTX101533). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SEPT7 antibody [N3C3] (GTX101533). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169