Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using 39332(GTX119118) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SEPT8 antibody detects SEPT8 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SEPT8 protein stained by SEPT8 antibody (GTX119118) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX119118 diluted at 1:2000

SEPT8 antibody detects SEPT8 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
SEPT8 antibody (GTX119118) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEPT8 antibody (GTX119118) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.