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SEPT9 antibody

Anti-SEPT9 antibody used in Flow cytometry (FACS). GTX04846
Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846
Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846
Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846
Anti-SEPT9 antibody used in Flow cytometry (FACS). GTX04846
Anti-SEPT9 antibody used in Western Blot (WB). GTX04846
Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846
Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846
Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846

Cat. No. GTX04846

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($469)
See all SEPT9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/ml
ICC/IF 5 μg/ml
IHC-P 2-5 μg/ml
FACS 1-3 μg/1x10⁶ cells
ELISA 0.1-0.5 μg/ml

Note :

IHC-P
Recommend EDTA buffer (pH 8.0) heat mediated antigen retrieval.
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human MSF/SEPTIN9 recombinant protein (Position: R15-D282).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AF17q25,MSF,MSF1,NAPB,PNUTL4,SEPT9,SINT1,SeptD1,septin 9,Septin 9

Cellular Localization

Cytoplasm,Cytoskeleton

Background

This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-SEPT9 antibody used in Flow cytometry (FACS). GTX04846

GTX04846 FACS Image

FACS analysis of 4% PFA-fixed HeLa cells using GTX04846 SEPT9 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Permeabilized step should be included.
Dilution : 1 μg/1x10⁶ cells

Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846

GTX04846 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX04846 SEPT9 antibody.
Antigen Retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/ml

Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846

GTX04846 IHC-P Image

IHC-P analysis of mouse lymphaden tissue using GTX04846 SEPT9 antibody.
Antigen Retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/ml

Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846

GTX04846 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX04846 SEPT9 antibody.
Antigen Retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 5 μg/ml

Anti-SEPT9 antibody used in Flow cytometry (FACS). GTX04846

GTX04846 FACS Image

FACS analysis of 4% PFA-fixed U251 cells using GTX04846 SEPT9 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Permeabilized step should be included.
Dilution : 1 μg/1x10⁶ cells

Anti-SEPT9 antibody used in Western Blot (WB). GTX04846

GTX04846 WB Image

WB analysis of various samples using GTX04846 SEPT9 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MOLT-4 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : Daudi whole cell lysate
Lane 6 : RT4 whole cell lysate
Lane 7 : SH-SY5Y whole cell lysate
Lane 8 : PC-3 whole cell lysate
Loading : 30 μg
Dilution : 0.5 μg/ml

Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846

GTX04846 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX04846 SEPT9 antibody.
Antigen Retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 5 μg/ml

Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846

GTX04846 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX04846 SEPT9 antibody.
Antigen Retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/ml

Anti-SEPT9 antibody used in IHC (Paraffin sections) (IHC-P). GTX04846

GTX04846 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX04846 SEPT9 antibody.
Antigen Retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 469