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SERCA1 ATPase antibody [VE121G9]

Anti-SERCA1 ATPase antibody [VE121G9] used in IHC (Paraffin sections) (IHC-P). GTX22819
Anti-SERCA1 ATPase antibody [VE121G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22819
Anti-SERCA1 ATPase antibody [VE121G9] used in IHC (Paraffin sections) (IHC-P). GTX22819
Anti-SERCA1 ATPase antibody [VE121G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22819
Anti-SERCA1 ATPase antibody [VE121G9] used in Western Blot (WB). GTX22819
Anti-SERCA1 ATPase antibody [VE121G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22819

Cat. No. GTX22819

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 VE121G9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Dog, Pig, Amphibians
Package
100 μl ($399)
See all SERCA1 ATPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF 1:500
IHC-P 1:20
IHC-Fr Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Product Note

This antibody detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase. It recognizes an epitope between amino acid residues 506 and the C-terminus of rabbit skeletal muscle ATPase, a region that is exposed in native sarcoplasmic reticulum.

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rabbit

Immunogen

Purified rabbit skeletal muscle sarcoplasmic reticulum.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 , ATP2A3 , SERCA1a

Cellular Localization

Endoplasmic reticulum membrane

Database

Research Area

DATA IMAGES

Anti-SERCA1 ATPase antibody [VE121G9] used in IHC (Paraffin sections) (IHC-P). GTX22819

GTX22819 IHC-P Image

IHC-P analysis of human heart tissue using GTX22819 SERCA1 ATPase antibody [VE121G9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-SERCA1 ATPase antibody [VE121G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22819

GTX22819 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22819 SERCA1 ATPase antibody [VE121G9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-SERCA1 ATPase antibody [VE121G9] used in IHC (Paraffin sections) (IHC-P). GTX22819

GTX22819 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX22819 SERCA1 ATPase antibody [VE121G9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-SERCA1 ATPase antibody [VE121G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22819

GTX22819 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX22819 SERCA1 ATPase antibody [VE121G9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-SERCA1 ATPase antibody [VE121G9] used in Western Blot (WB). GTX22819

GTX22819 WB Image

WB analysis of 25 ug of human skeletal muscle (lane 1), rat skeletal muscle (lane 2) and mouse skeletal muscle (lane 3) using GTX22819 SERCA1 ATPase antibody [VE121G9].
Dilution : 1:2000

Anti-SERCA1 ATPase antibody [VE121G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22819

GTX22819 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22819 SERCA1 ATPase antibody [VE121G9]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399