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SERCA2 ATPase antibody [2A7-A1]

Anti-SERCA2 ATPase antibody [2A7-A1] used in IHC (Paraffin sections) (IHC-P). GTX22861
Anti-SERCA2 ATPase antibody [2A7-A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22861
Anti-SERCA2 ATPase antibody [2A7-A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22861
Anti-SERCA2 ATPase antibody [2A7-A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22861
Anti-SERCA2 ATPase antibody [2A7-A1] used in IHC (Paraffin sections) (IHC-P). GTX22861
Anti-SERCA2 ATPase antibody [2A7-A1] used in IHC (Paraffin sections) (IHC-P). GTX22861
Anti-SERCA2 ATPase antibody [2A7-A1] used in Western Blot (WB). GTX22861

Cat. No. GTX22861

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A7-A1

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Drosophila, Dog, Guinea pig, Pig, Amphibians, Xenopus, Rodent
Package
100 μl ($399)
See all SERCA2 ATPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:100-1:250
IHC-P 1 μg/ml
IHC-Fr 1:100
FACS 1:20
IP Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Product Note

This antibody recognizes the SERCA2a and SERCA2b isoforms identically. However, the antibody does not recognize the skeletal muscle (SERCA1) or housekeeping isoforms (SERCA3).

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Dog

Immunogen

Purified canine cardiac sarcoplasmic reticulum vesicles.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384881

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 , SERCA2A

Cellular Localization

Endoplasmic reticulum membrane

Database

Research Area

DATA IMAGES

Anti-SERCA2 ATPase antibody [2A7-A1] used in IHC (Paraffin sections) (IHC-P). GTX22861

GTX22861 IHC-P Image

IHC-P analysis of human liver tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-SERCA2 ATPase antibody [2A7-A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22861

GTX22861 ICC/IF Image

ICC/IF analysis of A549 cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-SERCA2 ATPase antibody [2A7-A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22861

GTX22861 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-SERCA2 ATPase antibody [2A7-A1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22861

GTX22861 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

Anti-SERCA2 ATPase antibody [2A7-A1] used in IHC (Paraffin sections) (IHC-P). GTX22861

GTX22861 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-SERCA2 ATPase antibody [2A7-A1] used in IHC (Paraffin sections) (IHC-P). GTX22861

GTX22861 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-SERCA2 ATPase antibody [2A7-A1] used in Western Blot (WB). GTX22861

GTX22861 WB Image

WB analysis of whole cell extracts (30 μg lysate) of DU-145 (Lane 1), L6 (Lane 2), U-87MG (Lane 3) and PANC 1 (Lane 4) using GTX22861 SERCA2 ATPase antibody [2A7-A1].
Dilution : 1:1000

REFERENCE

Application Reference

Chung YW et al. Proc Natl Acad Sci U S A 2015; 112 (17) : E2253-62 Targeted disruption of PDE3B, but not PDE3A, protects murine heart from ischemia/reperfusion injury.
Application : IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399