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SERCA2 ATPase antibody [HL2352]

Recombinant antibody KO/KD Orthogonal
Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in IHC (Paraffin sections) (IHC-P). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in IHC (Paraffin sections) (IHC-P). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in IHC (Paraffin sections) (IHC-P). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555
Anti-SERCA2 ATPase antibody [HL2352] used in IHC (Paraffin sections) (IHC-P). GTX638555

Cat. No. GTX638555

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2352

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all SERCA2 ATPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SERCA2 ATPase. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 , ATP2B , DAR , DD , SERCA2

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555

GTX638555 WB Image

KO/KD

Non-transfected (–) and transfected (+) A549 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SERCA2 ATPase antibody [HL2352] used in IHC (Paraffin sections) (IHC-P). GTX638555

GTX638555 IHC-P Image

SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SERCA2 ATPase antibody [HL2352] used in IHC (Paraffin sections) (IHC-P). GTX638555

GTX638555 IHC-P Image

SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog tissues.
SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SERCA2 ATPase antibody [HL2352] used in IHC (Paraffin sections) (IHC-P). GTX638555

GTX638555 IHC-P Image

SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555

GTX638555 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555

GTX638555 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555

GTX638555 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555

GTX638555 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SERCA2 ATPase antibody [HL2352] used in Western Blot (WB). GTX638555

GTX638555 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SERCA2 ATPase antibody [HL2352] used in IHC (Paraffin sections) (IHC-P). GTX638555

GTX638555 IHC-P Image

SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations