SERCA2 ATPase antibody [IID8]

Cat. No. GTX22817

Host	Mouse
Clonality	Monoclonal
Clone Name	IID8
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA
Reactivity	Human, Mouse, Rat, Rabbit, Sheep, Bovine, Dog, Hamster, Guinea pig, Pig, Amphibians, Primate
Package	100 μl ($399)

See all SERCA2 ATPase products

Datasheet
Document

View product citations for antibody GTX22817 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:5000
ICC/IF	1:100-1:200
IHC-P	1 μg/ml
IHC-Fr	1:500
FACS	1μg per 10⁶ cells
IP	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Product Note

This antibody detects sarcoplasmic or endoplasmic reticulum calcium 2 (SERCA2) ATPase.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dog

Immunogen

Purified canine cardiac sarcoplasmic reticulum.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 , SERCA2A

Cellular Localization

Endoplasmic reticulum membrane

Database

Gene ID: 403878 ATP2A2
UniProt: O46674 ATP2A2

Research Area

DATA IMAGES

GTX22817 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

GTX22817 WB Image

WB analysis of 25 ug of rat skeletal muscle (lane 1) and human skeletal muscle (lane 2) using GTX22817 SERCA2 ATPase antibody [IID8].
Dilution : 1:1000 (rat muscle) and 1:2000 (human muscle)

GTX22817 ICC/IF Image

ICC/IF analysis of A549 cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

GTX22817 IHC-P Image

IHC-P analysis of human heart tissue using GTX22817 SERCA2 ATPase antibody [IID8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX22817 ICC/IF Image

ICC/IF analysis of U251 cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

GTX22817 IHC-P Image

IHC-P analysis of human liver tissue using GTX22817 SERCA2 ATPase antibody [IID8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX22817 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX22817 SERCA2 ATPase antibody [IID8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

SERCA2 ATPase antibody [IID8]

Cat. No. GTX22817

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Database

Research Area

DATA IMAGES

GTX22817 ICC/IF Image

GTX22817 WB Image

GTX22817 ICC/IF Image

GTX22817 IHC-P Image

GTX22817 ICC/IF Image

GTX22817 IHC-P Image

GTX22817 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.