GeneTex

  • Country / Location Selection

United States (US)

SERCA2 ATPase antibody

Anti-SERCA2 ATPase antibody used in Immunoprecipitation (IP). GTX23625
Anti-SERCA2 ATPase antibody used in Western Blot (WB). GTX23625

Cat. No. GTX23625

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Rabbit, Pig
Package
50 μl ($399)
See all SERCA2 ATPase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:5000
IP 1:10 - 1:500
ChIP assay 1:10 - 1:500
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

Predict Reactivity

Chicken, Xenopus laevis, Xenopus tropicalis, Chimpanzee, Gibbon, Gorilla, Orangutan, Panda, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen recognized by this antibody maps to a region between residue 475 and 525 of human ATPase, Ca++ transporter, cardiac muscle, slow twitch 2 using the numbering given in entry NP_733765.1 (GeneID 488).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_368179

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 , ATP2B , DAR , DD , SERCA2

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SERCA2 ATPase antibody used in Immunoprecipitation (IP). GTX23625

GTX23625 IP Image

IP analysis of 293T cell lysate using GTX23625 SERCA2 ATPase antibody.
IP protein : 0.5 -1.0 mg
Dilution : 1 μg/ml for WB ; 0.5-1 mg lysate / 6μg antibody for IP reaction
Loading: 20% of IP loaded

Anti-SERCA2 ATPase antibody used in Western Blot (WB). GTX23625

GTX23625 WB Image

WB analysis of HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 cell lysates (in NETN lysis buffer) using GTX23625 SERCA2 ATPase antibody.
Loading : 50μg
Dilution : 1 μg/ml

REFERENCE

Application Reference

Cantero-Recasens G et al. Hum Mol Genet 2010; 19 (1) : 111-21 The asthma-associated ORMDL3 gene product regulates endoplasmic reticulum-mediated calcium signaling and cellular stress.
Submit a Reference

REVIEW

There are currently no reviews for SERCA2 ATPase antibody (GTX23625). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399