APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , K562 , BCL-1
Predict Reactivity
Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human SERCA3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Atpase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 3,Serca3,Atp2A3
Cellular Localization
Nucleus membrane , Endoplasmic reticulum membrane , Sarcoplasmic reticulum membrane
Background
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Database
DATA IMAGES
|
GTX102381 WB Image
Sample (30 ug of whole cell lysate)
A: BCL-1
5% SDS PAGE
GTX102381 diluted at 1:5000
|
|
GTX102381 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SERCA3(GTX102381) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
GTX102381 WB Image
Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
5% SDS PAGE
GTX102381 diluted at 1:2000
|
