GeneTex
United States (US)

SERCA3 antibody [N1N2], N-term

GTX102381_40758_WB_M_19011506_317.jpg
GTX102381_40758_IHC_19011506_276.jpg
GTX102381_40758_WB_19011506_803.jpg

Cat No. GTX102381

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , BCL-1

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SERCA3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Atpase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 3,Serca3,Atp2A3

Cellular Localization

Nucleus membrane , Endoplasmic reticulum membrane , Sarcoplasmic reticulum membrane

Background

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

DATA IMAGES
GTX102381_40758_WB_M_19011506_317.jpg

GTX102381 WB Image

Sample (30 ug of whole cell lysate)
A: BCL-1
5% SDS PAGE
GTX102381 diluted at 1:5000

GTX102381_40758_IHC_19011506_276.jpg

GTX102381 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SERCA3(GTX102381) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102381_40758_WB_19011506_803.jpg

GTX102381 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: K562
5% SDS PAGE
GTX102381 diluted at 1:2000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169