Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA3 antibody [N1N2], N-term (GTX102381) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Jurkat whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SERCA3 antibody [N1N2], N-term (GTX102381) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SERCA3(GTX102381) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: BCL-1
5% SDS PAGE
GTX102381 diluted at 1:5000