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SERPINB6 antibody [N2C2], Internal

Anti-SERPINB6 antibody [N2C2], Internal used in Western Blot (WB). GTX114637
Anti-SERPINB6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114637
Anti-SERPINB6 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114637
Anti-SERPINB6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114637

Cat. No. GTX114637

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Bovine
Package
100 μl ($399),
25 μl ($169)
See all SERPINB6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SERPINB6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620608

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family B member 6 , CAP , DFNB91 , MSTP057 , PI-6 , PI6 , PTI , SPI3

Cellular Localization

Cytoplasm

Background

May be involved in the regulation of serine proteinases present in the brain or extravasated from the blood (By similarity). Inhibitor of cathepsin G, kallikrein-8 and thrombin. May play an important role in the inner ear in the protection against leakage of lysosomal content during stress and loss of this protection results in cell death and sensorineural hearing loss.

Database

Research Area

DATA IMAGES

Anti-SERPINB6 antibody [N2C2], Internal used in Western Blot (WB). GTX114637

GTX114637 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hela
10% SDS PAGE
GTX114637 diluted at 1:1000

Anti-SERPINB6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114637

GTX114637 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using SERPINB6 antibody(GTX114637) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SERPINB6 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114637

GTX114637 ICC/IF Image

SERPINB6 antibody [N2C2], Internal detects SERPINB6 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SERPINB6 protein stained by SERPINB6 antibody [N2C2], Internal (GTX114637) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SERPINB6 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114637

GTX114637 IHC-P Image

The data was published in the journal Reprod Biol Endocrinol in 2011. PMID: 21619581

REFERENCE

Application Reference

Hayashi KG et al. Reprod Biol Endocrinol 2011; 9 : 72 Differential gene expression of serine protease inhibitors in bovine ovarian follicle: possible involvement in follicular growth and atresia.
Application : IHC
Reactivity : Bovine
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169