Sample (50 ug of whole cell lysate)
A: mouse heart
10% SDS PAGE
GTX123522 diluted at 1:500

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody (GTX123522) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.