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SERPINE2 antibody

Orthogonal
Anti-SERPINE2 antibody used in Western Blot (WB). GTX124069
Anti-SERPINE2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124069
Anti-SERPINE2 antibody used in Western Blot (WB). GTX124069
Anti-SERPINE2 antibody used in Western Blot (WB). GTX124069
Anti-SERPINE2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124069
Anti-SERPINE2 antibody used in Western Blot (WB). GTX124069

Cat. No. GTX124069

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SERPINE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SERPINE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170075

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serpin family E member 2 , GDN , GDNPF , PI-7 , PI7 , PN-1 , PN1 , PNI

Cellular Localization

Secreted , extracellular space

Database

Research Area

DATA IMAGES

Anti-SERPINE2 antibody used in Western Blot (WB). GTX124069

GTX124069 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody (GTX124069) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SERPINE2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124069

GTX124069 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using SERPINE2(GTX124069) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SERPINE2 antibody used in Western Blot (WB). GTX124069

GTX124069 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody (GTX124069) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SERPINE2 antibody used in Western Blot (WB). GTX124069

GTX124069 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX124069 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SERPINE2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124069

GTX124069 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SERPINE2(GTX124069) antibody at 1:500 dilution.

Anti-SERPINE2 antibody used in Western Blot (WB). GTX124069

GTX124069 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SERPINE2 antibody (GTX124069) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shen Y et al. Cancer Biomark 2017; (Epub) SerpinE2, a poor biomarker of endometrial cancer, promotes the proliferation and mobility of EC cells.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169