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SESN3 antibody

Anti-SESN3 antibody used in Western Blot (WB). GTX112277
Anti-SESN3 antibody used in Western Blot (WB). GTX112277
Anti-SESN3 antibody used in Western Blot (WB). GTX112277
Anti-SESN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112277

Cat No. GTX112277

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HeLa , Raw264.7 , C2C12 , Rat2

Predict Reactivity

Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SESN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037973

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sestrin 3 , SEST3

Database

Research Area

DATA IMAGES

Anti-SESN3 antibody used in Western Blot (WB). GTX112277

GTX112277 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SESN3 antibody (GTX112277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SESN3 antibody used in Western Blot (WB). GTX112277

GTX112277 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody (GTX112277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SESN3 antibody used in Western Blot (WB). GTX112277

GTX112277 WB Image

SESN3 antibody detects SESN3 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
SESN3 antibody (GTX112277) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SESN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112277

GTX112277 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SESN3(GTX112277) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf