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SESN3 antibody

Orthogonal
Anti-SESN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112277
Anti-SESN3 antibody used in Western Blot (WB). GTX112277
Anti-SESN3 antibody used in Western Blot (WB). GTX112277
Anti-SESN3 antibody used in Western Blot (WB). GTX112277
Anti-SESN3 antibody used in Western Blot (WB). GTX112277

Cat. No. GTX112277

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SESN3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Rat2

Predict Reactivity

Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SESN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037973

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sestrin 3 , SEST3

Database

Research Area

DATA IMAGES

Anti-SESN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112277

GTX112277 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using SESN3(GTX112277) antibody at 1:200 dilution.

Anti-SESN3 antibody used in Western Blot (WB). GTX112277

GTX112277 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody (GTX112277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SESN3 antibody used in Western Blot (WB). GTX112277

GTX112277 WB Image

SESN3 antibody detects SESN3 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
SESN3 antibody (GTX112277) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SESN3 antibody used in Western Blot (WB). GTX112277

GTX112277 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SESN3 antibody (GTX112277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SESN3 antibody used in Western Blot (WB). GTX112277

GTX112277 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody (GTX112277) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169