APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89960-PEP).
Product Note
This antibody is expected to recognize isoform 2 (NP_003002.2).
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence SAPAAKVSKKELNS-C, from the N Terminus of the protein sequence according to NP_003002.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SET nuclear proto-oncogene , 2PP2A , I2PP2A , IGAAD , IPP2A2 , MRD58 , PHAPII , TAF-I , TAF-IBETA
Cellular Localization
Cytoplasm, cytosol , Endoplasmic reticulum , nucleoplasm
Background
The protein encoded by this gene inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The encoded protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Database
Research Area
DATA IMAGES
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GTX89960 WB Image
WB analysis of human kidney lysate using GTX89960 SET antibody, N-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89960 IHC-P Image
IHC-P analysis of human adrenal gland using GTX89960 SET antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89960 IHC-P Image
IHC-P analysis of human testis using GTX89960 SET antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89960 WB Image
WB analysis of Daudi (A) and Molt-4 (B) cell lysate using GTX89960 SET antibody, N-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for SET antibody, N-term (GTX89960). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SET antibody, N-term (GTX89960). Be the first to share your experience with this product.
