APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Product Note
This antibody is specific for SET isoform 2 (TAF-I beta) and does not cross with other SET isoforms.
Predict Reactivity
Mouse, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.96 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SET isoform 2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241317
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SET nuclear proto-oncogene , 2PP2A , I2PP2A , IGAAD , IPP2A2 , MRD58 , PHAPII , TAF-I , TAF-IBETA
Cellular Localization
Cytoplasm , cytosol , Endoplasmic reticulum , Nucleus , nucleoplasm
Database
Research Area
DATA IMAGES
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GTX106342 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc (GTX106342) diluted at 1:1000.
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GTX106342 ICC/IF Image
SET antibody, isoform 2-specific detects SET protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SET protein stained by SET antibody, isoform 2-specific (GTX106342) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.
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GTX106342 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SET antibody, isoform 2 specific (GTX106342) at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for SET antibody, isoform 2-specific (GTX106342). Be the first to share your publications with this product.
REVIEW
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