GeneTex
United States (US)

SET antibody, isoform 2-specific

GTX106342_39715_20160525_IFA_19011506_927.jpg
GTX106342_39715_20160428_WB_19011506_796.jpg
GTX106342_39715_IHC_19011506_893.jpg

Cat No. GTX106342

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Specificity/Sensitivity

This antibody is specific for SET isoform 2 (TAF-I beta) and does not cross with other SET isoforms.

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.959mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SET isoform 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

2PP2A antibody, I2PP2A antibody, IGAAD antibody, IPP2A2 antibody, PHAPII antibody, TAF-I antibody, TAF-IBETA antibody, SET antibody, inhibitor-2 of protein phosphatase-2A antibody, phosphatase 2A inhibitor I2PP2A antibody, inhibitor of granzyme A-activated DNase antibody, HLA-DR-associated protein II antibody, SET translocation (myeloid leukemia-associated) antibody, "Template-Activating Factor-I, chromatin remodelling factor antibody", protein SET antibody, protein phosphatase type 2A inhibitor antibody, SET nuclear oncogene antibody

Cellular Localization

Cytoplasm , cytosol , Endoplasmic reticulum , Nucleus , nucleoplasm

Database

Research Area

DATA IMAGES
GTX106342_39715_20160525_IFA_19011506_927.jpg

GTX106342 ICC/IF Image

SET antibody, isoform 2-specific detects SET protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SET protein stained by SET antibody, isoform 2-specific (GTX106342) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

GTX106342_39715_20160428_WB_19011506_796.jpg

GTX106342 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc (GTX106342) diluted at 1:1000.

GTX106342_39715_IHC_19011506_893.jpg

GTX106342 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SET antibody, isoform 2 specific (GTX106342) at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf