*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2
This antibody is specific for SET isoform 2 (TAF-I beta) and does not cross with other SET isoforms.
Bovine, Chicken, Pig(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SET isoform 2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
2PP2A antibody, I2PP2A antibody, IGAAD antibody, IPP2A2 antibody, PHAPII antibody, TAF-I antibody, TAF-IBETA antibody, SET antibody, inhibitor-2 of protein phosphatase-2A antibody, phosphatase 2A inhibitor I2PP2A antibody, inhibitor of granzyme A-activated DNase antibody, HLA-DR-associated protein II antibody, SET translocation (myeloid leukemia-associated) antibody, "Template-Activating Factor-I, chromatin remodelling factor antibody", protein SET antibody, protein phosphatase type 2A inhibitor antibody, SET nuclear oncogene antibody
Cytoplasm , cytosol , Endoplasmic reticulum , Nucleus , nucleoplasm
GTX106342 ICC/IF Image
SET antibody, isoform 2-specific detects SET protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SET protein stained by SET antibody, isoform 2-specific (GTX106342) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.
GTX106342 WB Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody, isoform 2-specifc (GTX106342) diluted at 1:1000.
GTX106342 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SET antibody, isoform 2 specific (GTX106342) at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min