APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Observed MW
42/44 kDa. The observed M.W. is based on the following publication. PMID: 9597000
Positive Control
A431 , H1299 , HeLa , HepG2 , Molt-4 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2
Product Note
Knockdown/Knockout validation was supported by customer review data.
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SET. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2037974
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SET nuclear proto-oncogene , 2PP2A , I2PP2A , IGAAD , IPP2A2 , MRD58 , PHAPII , TAF-I , TAF-IBETA
Cellular Localization
Cytoplasm , cytosol , Endoplasmic reticulum , Nucleus , nucleoplasm
Database
Research Area
DATA IMAGES
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GTX113834 WB Image
SET antibody detects SET protein by Western blot analysis.
A. 30 μg A431 whole cell lysate/extract whole cell lysate/extract
B. 30 μg H1299 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg Molt-4 whole cell lysate/extract
F. 30 μg Raji whole cell lysate/extract
12 % SDS-PAGE
SET antibody (GTX113834) dilution: 1:1000 SET antibody detects SET protein by Western blot analysis.
Observed SET protein is larger than the predicted M.W., possibly due to post-translational modifications.
Reference: Exp Cell Res. 1998 May 1;240(2):274-81.
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GTX113834 WB Image
Non-transfected (–) and transfected (+) C2C12 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody (GTX113834) diluted at 1:1000.
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GTX113834 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody (GTX113834) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113834 WB Image
SET antibody detects SET protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
SET antibody (GTX113834) dilution: 1:1000
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GTX113834 IP Image
SET antibody immunoprecipitates SET protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SET protein by 4 μg SET antibody (GTX113834)
10 % SDS-PAGE
The immunoprecipitated SET protein was detected by SET antibody (GTX113834) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX113834 ICC/IF Image
SET antibody detects SET protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SET protein stained by SET antibody (GTX113834) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX113834 WB Image
SET antibody detects SET protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw 264.7 whole cell lysate/extract
G. 30 μg C2Cl2 whole cell lysate/extract
12 % SDS-PAGE
SET antibody (GTX113834) dilution: 1:1000
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REFERENCE
REVIEW
SET antibody
Cat. No. GTX113834
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 26-Apr-2016
Application Tested :
WB
Sample Species :
Ms
Sample :
C2C12 cells
Amount used :
40 μg
Blocking :
1% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Lane Description :
Lane 1: C2C12 cells, Lane 2-4: knockdown of SET in C2C12 cells
