Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody [HL2705] (GTX639459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

SETD1A antibody [HL2705] detects SETD1A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETD1A stained by SETD1A antibody [HL2705] (GTX639459) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody [HL2705] (GTX639459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.