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SETD1A antibody [HL2705]

Recombinant antibody Orthogonal
Anti-SETD1A antibody [HL2705] used in Western Blot (WB). GTX639459
Anti-SETD1A antibody [HL2705] used in Western Blot (WB). GTX639459
Anti-SETD1A antibody [HL2705] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639459

Cat. No. GTX639459

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2705

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SETD1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

186 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human SETD1A.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET domain containing 1A, histone lysine methyltransferase , KMT2F , Set1 , Set1A

Cellular Localization

Nucleus speckle

Background

The protein encoded by this gene is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-SETD1A antibody [HL2705] used in Western Blot (WB). GTX639459

GTX639459 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody [HL2705] (GTX639459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SETD1A antibody [HL2705] used in Western Blot (WB). GTX639459

GTX639459 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETD1A antibody [HL2705] (GTX639459) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-SETD1A antibody [HL2705] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639459

GTX639459 ICC/IF Image

SETD1A antibody [HL2705] detects SETD1A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETD1A stained by SETD1A antibody [HL2705] (GTX639459) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations