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SETD7 antibody

Anti-SETD7 antibody used in IHC (Paraffin sections) (IHC-P). GTX13731
Anti-SETD7 antibody used in Western Blot (WB). GTX13731

Cat. No. GTX13731

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
200 μl ($399)
See all SETD7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100
ChIP assay 1:10 - 1:500
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This is specific for Set7/9 (a histone H3 lysine 4 methyltransferase) and does not recognize PR-Set7 (a histone H4 lysine 20 methyltransferase).

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Two synthethic peptides corresponding to amino acids 131-145 (GEVNEDGEMTGEKIA) and 336-352 (GYDHSPPGKSGPEAPEW) of human SETD7 were used as immunogen for this antibody.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET domain containing 7, histone lysine methyltransferase , KMT7 , SET7 , SET7/9 , SET9

Cellular Localization

Nucleus

Background

Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Also able to demethylated 'Lys-372' of p53/TP53 in vitro.

Database

Research Area

DATA IMAGES

Anti-SETD7 antibody used in IHC (Paraffin sections) (IHC-P). GTX13731

GTX13731 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX13731 SETD7 antibody.
Dilution : 1:100

Anti-SETD7 antibody used in Western Blot (WB). GTX13731

GTX13731 WB Image

WB analysis of (A) MCF-7 and (B) HeLa cell lysate using GTX13731 SETD7 antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399