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SETD8 antibody

Anti-SETD8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119440
Anti-SETD8 antibody used in Western Blot (WB). GTX119440

Cat. No. GTX119440

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Caenorhabditis elegans
Package
100 μl ($399),
25 μl ($169)
See all SETD8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SETD8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine methyltransferase 5A , PR-Set7 , PR/SET07 , SET07 , SET8 , SETD8

Cellular Localization

Nucleus

Background

Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20' monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.

Database

Research Area

DATA IMAGES

Anti-SETD8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119440

GTX119440 ICC/IF Image

SETD8 antibody detects SETD8 protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETD8 protein stained by SETD8 antibody (GTX119440) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SETD8 antibody used in Western Blot (WB). GTX119440

GTX119440 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SETD8 antibody (GTX119440) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lu J et al. Nat Neurosci 2019; 22 (6) : 875-886 L3MBTL1 regulates ALS/FTD-associated proteotoxicity and quality control.
Application : WB
Reactivity : Caenorhabditis elegans
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169