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SETDB1 antibody [HL1645]

Recombinant antibody Comparison Orthogonal
Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226
Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226
Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226
Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226
Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226

Cat. No. GTX637226

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1645

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SETDB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SETDB1 The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET domain bifurcated histone lysine methyltransferase 1 , ESET , H3-K9-HMTase4 , KG1T , KMT1E , TDRD21

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226

GTX637226 WB Image

Comparison Orthogonal

HeLa whole cell and nuclear extract (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226

GTX637226 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226

GTX637226 ICC/IF Image

SETDB1 antibody [HL1645] detects SETDB1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETDB1 stained by SETDB1 antibody [HL1645] (GTX637226) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226

GTX637226 WB Image

Various tissue extracts (50 Âμg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226

GTX637226 IHC-P Image

SETDB1 antibody [HL1645] detects SETDB1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SETDB1 stained by SETDB1 antibody [HL1645] (GTX637226) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations