APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SETDB1 The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
SET domain bifurcated histone lysine methyltransferase 1 , ESET , H3-K9-HMTase4 , KG1T , KMT1E , TDRD21
Cellular Localization
Nucleus,Cytoplasm
Background
This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011]
Database
Research Area
DATA IMAGES
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/templates/default/js/lightbox/images/loading.gif "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
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GTX637226 WB Image
Various tissue extracts (50 Âμg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637226 IHC-P Image
SETDB1 antibody [HL1645] detects SETDB1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.SETDB1 stained by SETDB1 antibody [HL1645] (GTX637226) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637226 WB Image
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637226 ICC/IF Image
SETDB1 antibody [HL1645] detects SETDB1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [HL1645] (GTX637226) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for SETDB1 antibody [HL1645] (GTX637226). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SETDB1 antibody [HL1645] (GTX637226). Be the first to share your experience with this product.
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_T-44746_20220805_WB_M_R_22080923_527.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_T-44746_20220819_IHC-P_M_22083119_273.webp "Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_44830_20221014_WB_Fraction_22101718_496.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_T-44746_20220902_ICC_IF_22101718_996.webp "Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_T-44746_20220805_WB_M_R_22080923_527.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_T-44746_20220819_IHC-P_M_22083119_273.webp "Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_44830_20221014_WB_Fraction_22101718_496.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_T-44746_20220902_ICC_IF_22101718_996.webp "Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226")