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SETDB1 antibody [N1], N-term

Orthogonal
Anti-SETDB1 antibody [N1], N-term used in Western Blot (WB). GTX115305
Anti-SETDB1 antibody [N1], N-term used in Western Blot (WB). GTX115305
Anti-SETDB1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX115305
Anti-SETDB1 antibody [N1], N-term used in ChIP assay (ChIP assay). GTX115305

Cat. No. GTX115305

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SETDB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SETDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176036

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET domain bifurcated histone lysine methyltransferase 1 , ESET , H3-K9-HMTase4 , KG1T , KMT1E , TDRD21

Cellular Localization

Nucleus

Background

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

Database

Research Area

DATA IMAGES

Anti-SETDB1 antibody [N1], N-term used in Western Blot (WB). GTX115305

GTX115305 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SETDB1 antibody [N1], N-term used in Western Blot (WB). GTX115305

GTX115305 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SETDB1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX115305

GTX115305 IP Image

SETDB1 antibody [N1], N-term immunoprecipitates SETDB1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of SETDB1 protein by 4 μg SETDB1 antibody [N1], N-term (GTX115305)
5 % SDS-PAGE
The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-SETDB1 antibody [N1], N-term used in ChIP assay (ChIP assay). GTX115305

GTX115305 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to P53P2.
ChIP experiment and primer designs are based on J Biol Chem.?2006 Jul 14;281(28):19489-500.?

REFERENCE

Application Reference

Wu K et al. Cell Rep 2020; 30 (1) : 25-36.e6 SETDB1-Mediated Cell Fate Transition between 2C-Like and Pluripotent States.
Application : WB, ChIP assay
Reactivity : Mouse
Ho YJ et al. APMIS 2017; (Epub) Significance of histone methyltransferase SETDB1 expression in colon adenocarcinoma.
Application : IHC-P
Reactivity : Human
Chen J et al. Nat Genet 2013; 45 (1) : 34-42 H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Application : IP
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169