GeneTex
United States (US)

SETDB2 antibody [N1], N-term

GTX108086_39728_IFA_19011507_352.jpg

Cat No. GTX108086

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:2000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SETDB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Set Domain Bifurcated 2,C13Orf4,Clld8,Clll8,Kmt1F,Setdb2

Cellular Localization

Nucleus

Background

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX108086_39728_IFA_19011507_352.jpg

GTX108086 ICC/IF Image

SETDB2 antibody [N1], N-term detects SETDB2 protein at nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETDB2 protein stained by SETDB2 antibody [N1], N-term (GTX108086) diluted at 1:2000.
Blue: Hoechst 33342 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169