SETDB2 antibody [N1N2], N-term

Cat. No. GTX116932

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all SETDB2 products

Datasheet
Document

View product citations for antibody GTX116932 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

SK-N-SH

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SETDB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169569

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SET domain bifurcated histone lysine methyltransferase 2 , C13orf4 , CLLD8 , CLLL8 , KMT1F

Cellular Localization

Nucleus

Background

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM]

Database

Gene ID: 83852 SETDB2
UniProt: Q96T68 SETDB2

Research Area

DATA IMAGES

GTX116932 IP Image

SETDB2 antibody immunoprecipitates SETDB2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SETDB2 protein by 2 μg of SETDB2 antibody (GTX116932) 7.5% SDS-PAGE The immunoprecipitated SETDB2 protein was detected by SETDB2 antibody (GTX116932) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX116932 ICC/IF Image

SETDB2 antibody [N1N2], N-term detects SETDB2 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SETDB2 protein stained by SETDB2 antibody [N1N2], N-term (GTX116932) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX116932 WB Image

Sample (30 ug of whole cell lysate)
A: SK-N-SH
7.5% SDS PAGE
GTX116932 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty