GeneTex
United States (US)

SF2 antibody

GTX114918_40240_IHC_M_2_18121410_239.jpg
GTX114918_40464_20160114_WB_18121410_808.jpg
GTX114918_40240_IHC_M_18121410_506.jpg
GTX114918_40240_IHC_18121410_335.jpg
GTX114918_40240_IFA_18121410_336.jpg
GTX114918_40240_IHC_2_18121410_775.jpg
GTX114918_40464_20170901_WB_R_brain_18121410_349.jpg
GTX114918_40240_WB_M_brain_18121410_522.jpg

Cat No. GTX114918

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

rat brain , HeLa , HepG2 , Mouse brain

Predict Reactivity

Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Serine And Arginine Rich Splicing Factor 1,Asf,Sf2,Sf2P33,Sfrs1,Srp30A,Srsf1

Cellular Localization

Cytoplasm , Nucleus speckle

Background

This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX114918_40240_IHC_M_2_18121410_239.jpg

GTX114918 IHC-P Image

SF2 antibody detects SF2 protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SF2 antibody (GTX114918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114918_40464_20160114_WB_18121410_808.jpg

GTX114918 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody (GTX114918) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114918_40240_IHC_M_18121410_506.jpg

GTX114918 IHC-P Image

SF2 antibody detects SF2 protein at nucleus on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
SF2 antibody (GTX114918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114918_40240_IHC_18121410_335.jpg

GTX114918 IHC-P Image

SF2 antibody detects SFRS1 protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
SF2 antibody (GTX114918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114918_40240_IFA_18121410_336.jpg

GTX114918 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SF2(GTX114918) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX114918_40240_IHC_2_18121410_775.jpg

GTX114918 IHC-P Image

SF2 antibody detects SFRS1 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
SF2 antibody (GTX114918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114918_40464_20170901_WB_R_brain_18121410_349.jpg

GTX114918 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody (GTX114918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114918_40240_WB_M_brain_18121410_522.jpg

GTX114918 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114918 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169