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SF2 antibody

Anti-SF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114918
Anti-SF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114918
Anti-SF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114918
Anti-SF2 antibody used in Western Blot (WB). GTX114918
Anti-SF2 antibody used in Western Blot (WB). GTX114918
Anti-SF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114918
Anti-SF2 antibody used in Western Blot (WB). GTX114918
Anti-SF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114918

Cat. No. GTX114918

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brain , HeLa , HepG2 , rat brain

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

serine and arginine rich splicing factor 1 , ASF , SF2 , SF2p33 , SFRS1 , SRp30a

Cellular Localization

Cytoplasm , Nucleus speckle

Background

This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114918

GTX114918 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SF2(GTX114918) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114918

GTX114918 IHC-P Image

SF2 antibody detects SF2 protein at nucleus on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
SF2 antibody (GTX114918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114918

GTX114918 IHC-P Image

SF2 antibody detects SFRS1 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
SF2 antibody (GTX114918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SF2 antibody used in Western Blot (WB). GTX114918

GTX114918 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody (GTX114918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SF2 antibody used in Western Blot (WB). GTX114918

GTX114918 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114918 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114918

GTX114918 IHC-P Image

SF2 antibody detects SFRS1 protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
SF2 antibody (GTX114918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SF2 antibody used in Western Blot (WB). GTX114918

GTX114918 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody (GTX114918) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114918

GTX114918 IHC-P Image

SF2 antibody detects SF2 protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SF2 antibody (GTX114918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Wang J et al. mBio 2020; 11 (2) RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing Enhancers.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169