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SF3A1 antibody, N-term

Anti-SF3A1 antibody, N-term used in Western Blot (WB). GTX47357
Anti-SF3A1 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47357
Anti-SF3A1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47357

Cat. No. GTX47357

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
50 μg ($409)
See all SF3A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
ICC/IF Assay dependent
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

human B lymphoblast

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human SF3A1

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 3a subunit 1 , PRP21 , PRPF21 , SAP114 , SF3A120

Cellular Localization

Nucleus,Nucleus speckle

Background

This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing. [provided by RefSeq, Aug 2014]

Database

Research Area

DATA IMAGES

Anti-SF3A1 antibody, N-term used in Western Blot (WB). GTX47357

GTX47357 WB Image

WB analysis of human B lymphoblast cells using GTX47357 SF3A1 antibody at 0.2-1μg/ml.

Anti-SF3A1 antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47357

GTX47357 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX47357 SF3A1 antibody at 1:250. Left to right : DAPI, SF3A1, merge. Fix cells using 4% PFA for 30 mins. Wash 3x with permeabilizing wash buffer (PBS+0.3% Tx-100.). Block with 5% goat serum, 1% BSA, and 0.2% fish gelatin. Incubate at 4 ºC overnight.

Anti-SF3A1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47357

GTX47357 IHC-P Image

IHC-P analysis of human heart tissue using GTX47357 SF3A1 antibody at 4.0-8.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409