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SF3A3 antibody

Anti-SF3A3 antibody used in Western Blot (WB). GTX118225
Anti-SF3A3 antibody used in Western Blot (WB). GTX118225

Cat. No. GTX118225

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SF3A3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

Jurkat , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SF3A3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618487

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 3a subunit 3 , PRP9 , PRPF9 , SAP61 , SF3a60

Cellular Localization

Nucleus speckle

Background

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. [provided by RefSeq]

Database

DATA IMAGES

Anti-SF3A3 antibody used in Western Blot (WB). GTX118225

GTX118225 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX118225 diluted at 1:1000

Anti-SF3A3 antibody used in Western Blot (WB). GTX118225

GTX118225 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
7.5% SDS PAGE
GTX118225 diluted at 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169