APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
splicing factor 3b subunit 1 , Hsh155 , MDS , PRP10 , PRPF10 , SAP155 , SF3B1 , SF3b155
Cellular Localization
Nucleus,Nucleus speckle
Background
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165](/templates/default/js/lightbox/images/loading.gif "Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165")
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GTX01165 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SF3B1 antibody [JB40-32] (GTX01165) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX01165 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SF3B1 antibody [JB40-32] (GTX01165) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01165 IHC-P Image
IHC-P analysis of human colon cancer tissue section using GTX01165 SF3B1 antibody [JB40-32].
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GTX01165 IHC-P Image
IHC-P analysis of human kidney tissue section using GTX01165 SF3B1 antibody [JB40-32].
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GTX01165 IHC-P Image
IHC-P analysis of human skin tissue section using GTX01165 SF3B1 antibody [JB40-32].
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GTX01165 IHC-P Image
IHC-P analysis of mouse small intestine tissue section using GTX01165 SF3B1 antibody [JB40-32].
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GTX01165 IHC-P Image
IHC-P analysis of rat brain tissue section using GTX01165 SF3B1 antibody [JB40-32].
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REFERENCE
There are currently no references for SF3B1 antibody [JB40-32] (GTX01165). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SF3B1 antibody [JB40-32] (GTX01165). Be the first to share your experience with this product.
![Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165](/upload/website/prouct_img/small/GTX01165/GTX01165_HL0222_20200228_WB_TPM_watermark_20030122_863.jpg "Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165](/upload/website/prouct_img/small/GTX01165/GTX01165_HL0222_20200828_WB_M_thymus_20090323_818.jpg "Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/small/GTX01165/GTX01165_20200303_IHC-P_650_20030422_666.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/small/GTX01165/GTX01165_20200303_IHC-P_652_20030422_749.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/small/GTX01165/GTX01165_20200303_IHC-P_651_20030422_797.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/small/GTX01165/GTX01165_20200303_IHC-P_653_20030422_473.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/small/GTX01165/GTX01165_20200303_IHC-P_649_20030422_337.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165](/upload/website/prouct_img/normal/GTX01165/GTX01165_HL0222_20200228_WB_TPM_watermark_20030122_863.jpg "Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165](/upload/website/prouct_img/normal/GTX01165/GTX01165_HL0222_20200828_WB_M_thymus_20090323_818.jpg "Anti-SF3B1 antibody [JB40-32] used in Western Blot (WB). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/normal/GTX01165/GTX01165_20200303_IHC-P_650_20030422_666.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/normal/GTX01165/GTX01165_20200303_IHC-P_652_20030422_749.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/normal/GTX01165/GTX01165_20200303_IHC-P_651_20030422_797.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/normal/GTX01165/GTX01165_20200303_IHC-P_653_20030422_473.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")
![Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165](/upload/website/prouct_img/normal/GTX01165/GTX01165_20200303_IHC-P_649_20030422_337.jpg "Anti-SF3B1 antibody [JB40-32] used in IHC (Paraffin sections) (IHC-P). GTX01165")