SF3B1 antibody [JB40-32]

Cat. No. GTX01165

Host	Rabbit
Clonality	Monoclonal
Clone Name	JB40-32
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all SF3B1 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
IHC-P	1:50-1:200

Not tested in other applications.

Calculated MW

146 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 3b subunit 1 , Hsh155 , MDS , PRP10 , PRPF10 , SAP155 , SF3B1 , SF3b155

Cellular Localization

Nucleus,Nucleus speckle

Background

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Gene ID: 23451 SF3B1
UniProt: O75533 SF3B1

Research Area

DATA IMAGES

GTX01165 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SF3B1 antibody [JB40-32] (GTX01165) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX01165 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SF3B1 antibody [JB40-32] (GTX01165) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX01165 IHC-P Image

IHC-P analysis of human skin tissue section using GTX01165 SF3B1 antibody [JB40-32].

GTX01165 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX01165 SF3B1 antibody [JB40-32].

GTX01165 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX01165 SF3B1 antibody [JB40-32].

GTX01165 IHC-P Image

IHC-P analysis of human colon cancer tissue section using GTX01165 SF3B1 antibody [JB40-32].

GTX01165 IHC-P Image

IHC-P analysis of mouse small intestine tissue section using GTX01165 SF3B1 antibody [JB40-32].

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty

SF3B1 antibody [JB40-32]

Cat. No. GTX01165

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX01165 WB Image

GTX01165 WB Image

GTX01165 IHC-P Image

GTX01165 IHC-P Image

GTX01165 IHC-P Image

GTX01165 IHC-P Image

GTX01165 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.