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SF3B1 antibody, N-term

Anti-SF3B1 antibody, N-term used in Western Blot (WB). GTX47327
Anti-SF3B1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47327

Cat No. GTX47327

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

146 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human SF3B1

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

splicing factor 3b subunit 1 , Hsh155 , MDS , PRP10 , PRPF10 , SAP155 , SF3b155

Cellular Localization

Nucleus,Nucleus speckle

Background

This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SF3B1 antibody, N-term used in Western Blot (WB). GTX47327

GTX47327 WB Image

WB analysis of Jurkat cells using GTX47327 SF3B1 antibody at 0.3125μg/ml.
Lane A : marker
Lane B : Jurkat cells

Anti-SF3B1 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47327

GTX47327 IHC-P Image

IHC-P analysis of human heart tissue using GTX47327 SF3B1 antibody at 1:100. Left to right : DAPI, SF3B1, Merge. The low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf