APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.2-2.5 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
PROPERTIES
Form
Liquid
Buffer
1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human SF3B1
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
splicing factor 3b subunit 1 , Hsh155 , MDS , PRP10 , PRPF10 , SAP155 , SF3b155
Cellular Localization
Nucleus,Nucleus speckle
Background
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX47328 WB Image
WB analysis of HeLa cells using GTX47328 SF3B1 antibody at 0.2-1μg/ml.
|
REFERENCE
There are currently no references for SF3B1 antibody, N-term (GTX47328). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SF3B1 antibody, N-term (GTX47328). Be the first to share your experience with this product.
![Anti-NOTCH1 antibody [GT1099] used in Western Blot (WB). GTX64291](/upload/website/prouct_img/small/GTX64291/GTX64291_HL0222_20200228_WB_TPM_watermark_20030122_676.jpg "Anti-NOTCH1 antibody [GT1099] used in Western Blot (WB). GTX64291")
