GeneTex
United States (US)

SF3B4 antibody [N1], N-term

GTX106389_39757_IFA_19011506_979.jpg
GTX106389_WB_19011506_738.jpg
GTX106389_39757_WB_M_19011506_847.jpg

Cat No. GTX106389

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HeLa , NIH-3T3 , JC

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SF3B4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hsh49 antibody, MGC10828 antibody, SAP49 antibody, SF3b49 antibody, SF3B4 antibody, pre-mRNA-splicing factor SF3b 49 kDa subunit antibody, pre-mRNA splicing factor SF3b 49 kDa subunit antibody, SF3b50 antibody, spliceosome-associated protein 49 antibody, splicing factor 3B subunit 4 antibody, spliceosomal protein antibody, spliceosome-associated protein (U2 snRNP) antibody, SAP 49 antibody, "splicing factor 3b, subunit 4, 49kDa antibody"

Cellular Localization

Nucleus

Background

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq]
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Database

DATA IMAGES
GTX106389_39757_IFA_19011506_979.jpg

GTX106389 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SF3B4(GTX106389) antibody at 1:200 dilution.

GTX106389_WB_19011506_738.jpg

GTX106389 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX106389 diluted at 1:1000

GTX106389_39757_WB_M_19011506_847.jpg

GTX106389 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX106389 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169