Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SF3B4 antibody (GTX117942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: Jurkat
10% SDS PAGE
GTX117942 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX117942 diluted at 1:1000