Immunoprecipitation of SFPQ protein from 293T whole cell extracts using 5 μg of SFPQ antibody [N2C1], Internal (GTX114209).
Western blot analysis was performed using SFPQ antibody [N2C1], Internal (GTX114209).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded mouse uterus, using SFPQ(GTX114209) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SFPQ antibody [N2C1], Internal detects SFPQ protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SFPQ(GTX114209) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SFPQ antibody detects SFPQ protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody (GTX114209) diluted by 1:10000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min