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SFPQ antibody [N2C1], Internal

Anti-SFPQ antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX114209
Anti-SFPQ antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114209
Anti-SFPQ antibody [N2C1], Internal used in Western Blot (WB). GTX114209
Anti-SFPQ antibody [N2C1], Internal used in Western Blot (WB). GTX114209
Anti-SFPQ antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114209
Anti-SFPQ antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114209
Anti-SFPQ antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114209
Anti-SFPQ antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114209

Cat. No. GTX114209

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SFPQ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SFPQ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179808

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor proline and glutamine rich , POMP100 , PPP1R140 , PSF

Cellular Localization

Nucleus matrix

Database

Research Area

DATA IMAGES

Anti-SFPQ antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX114209

GTX114209 IP Image

Immunoprecipitation of SFPQ protein from 293T whole cell extracts using 5 μg of SFPQ antibody [N2C1], Internal (GTX114209).
Western blot analysis was performed using SFPQ antibody [N2C1], Internal (GTX114209).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SFPQ antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114209

GTX114209 IHC-P Image

SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SFPQ antibody [N2C1], Internal used in Western Blot (WB). GTX114209

GTX114209 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SFPQ antibody [N2C1], Internal used in Western Blot (WB). GTX114209

GTX114209 WB Image

SFPQ antibody detects SFPQ protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody (GTX114209) diluted by 1:10000.

Anti-SFPQ antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114209

GTX114209 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SFPQ(GTX114209) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SFPQ antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114209

GTX114209 IHC-P Image

SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SFPQ antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114209

GTX114209 ICC/IF Image

SFPQ antibody [N2C1], Internal detects SFPQ protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-SFPQ antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX114209

GTX114209 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse uterus, using SFPQ(GTX114209) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Gao Z et al. RNA Biol 2019; 16 (7) : 940-949 A novel human lncRNA SANT1 cis-regulates the expression of SLC47A2 by altering SFPQ/E2F1/HDAC1 binding to the promoter region in renal cell carcinoma.
Raab JR et al. Nat Genet 2019; 51 (1) : 26-29 SWI/SNF remains localized to chromatin in the presence of SCHLAP1.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169