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SFRP1 antibody

Anti-SFRP1 antibody used in Western Blot (WB). GTX24193
Anti-SFRP1 antibody used in Immunohistochemistry (IHC). GTX24193
Anti-SFRP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24193
Anti-SFRP1 antibody used in ELISA (ELISA). GTX24193

Cat. No. GTX24193

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, IHC

Reactivity

 Human
Package
50 μg ($399)
See all SFRP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:2000
ICC/IF 5 μg/mL
IHC-P 1:800
ELISA 1:5000-1:15000
IHC 1:800
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to a 12 aa region of human Sfrp1 protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_370619

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted frizzled related protein 1 , FRP , FRP-1 , FRP1 , FrzA , SARP2

Cellular Localization

Secreted

Background

SFRP1 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP1 and SFRP5 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP1 is expressed in several human tissues, with the highest levels in heart.

Database

Research Area

DATA IMAGES

Anti-SFRP1 antibody used in Western Blot (WB). GTX24193

GTX24193 WB Image

Western blot using GeneTex Affinity Purified anti-SFRP1 antibody (GTX24193) shows detection of a band ~37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4ºC. Signal was detected using standard techniques.

Anti-SFRP1 antibody used in Immunohistochemistry (IHC). GTX24193

GTX24193 IHC Image

GeneTex Affinity Purified anti-Human SFRP1 antibody (GTX24193) was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG (GTX26721) is suitable for secondary antibody detection.

Anti-SFRP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX24193

GTX24193 IHC-P Image

IHC-P analysis of human dermal hypertrophic scar tissue using GTX24193 SFRP1 antibody.
Antigen retrieval : Heat mediated buffer
Dilution : 1:800

Anti-SFRP1 antibody used in ELISA (ELISA). GTX24193

GTX24193 ELISA Image

ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX24193 SFRP1 antibody.
Coating : 0.1 μg

REFERENCE

Application Reference

Cheng LC et al. Sci Rep 2020; 10 (1) : 13255 Increased expression of secreted frizzled related protein 1 (SFRP1) predicts ampullary adenocarcinoma recurrence.
Application : IHC-P
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399