ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX24193 SFRP1 antibody.
Coating : 0.1 μg

IHC-P analysis of human dermal hypertrophic scar tissue using GTX24193 SFRP1 antibody.
Antigen retrieval : Heat mediated buffer
Dilution : 1:800

GeneTex Affinity Purified anti-Human SFRP1 antibody (GTX24193) was used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG (GTX26721) is suitable for secondary antibody detection.

Western blot using GeneTex Affinity Purified anti-SFRP1 antibody (GTX24193) shows detection of a band ~37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4ºC. Signal was detected using standard techniques.